abstract：:As an important direct solvent for cellulose, N-methyl morpholine N-oxide (NMMO) is environmentally friendly, and potentially very economical. Silk fibroin (SF) (Bombyx mori) can also be dissolved directly in NMMO.H2O. However, it is unexpectedly difficult to obtain a silk fibroin solution with a concentration higher ...

journal_title：International journal of biological macromolecules

authors： Xu Y,Zhang Y,Shao H,Hu X

更新日期：2005-04-01 00:00:00

abstract：:We have found that polyacrylamide gels cross-linked with bisacrylamide induce a larger macromolecular osmotic pressure than the non cross-linked gels. This means also that in the cross-linked gels hydration water is held more tightly to the polymer, i.e., more work is needed to remove water, than in the non cross-link...

journal_title：International journal of biological macromolecules

authors： Trombetta G,Di Bona C,Grazi E

更新日期：2005-03-01 00:00:00

abstract：:Laccase is one of a few enzymes that can directly reduce oxygen into water under ambient conditions, while oxidizing a variety of aromatic compounds. Its conjugation with chitosan generates a pH-sensitive functional biomaterial that changes its solubility in response to pH variation. The molecular conjugation between ...

journal_title：International journal of biological macromolecules

authors： Delanoy G,Li Q,Yu J

更新日期：2005-03-01 00:00:00

abstract：:Two kinds of water-insoluble (1-->3)-alpha-D-glucan samples, ab-PCM3-I and ac-PCM3-I, isolated from different Poria cocos mycelia were sulfated, to produce two series of water-soluble derivatives ab-PCM3-I-S1-S5 and ac-PCM3-I-S1-S5, respectively. The derivatives having different weight-average molecular mass (Mw) were...

journal_title：International journal of biological macromolecules

authors： Lin Y,Zhang L,Chen L,Jin Y,Zeng F,Jin J,Wan B,Cheung PC

更新日期：2004-10-01 00:00:00

abstract：:Stabilization of type I rat tail tendon (RTT) collagen by various aldehydes, viz. formaldehyde, gluteraldehyde, glyoxal and crotanaldehyde was studied to understand the effect of each on the thermal, enzymatic and conformational stability of collagen. The aldehydes have been found to increase the heat stability of rat...

journal_title：International journal of biological macromolecules

authors： Fathima NN,Madhan B,Rao JR,Nair BU,Ramasami T

更新日期：2004-08-01 00:00:00

abstract：:The corynemycolyltransferase proteins were identified from Corynebacterium glutamicum and Corynebacterium efficiens genomes using computational tools available in the public domain. Three-dimensional models were constructed for corynemycolyltransferases based on the crystal structures of related mycolyltransferases in...

journal_title：International journal of biological macromolecules

authors： Adindla S,Guruprasad K,Guruprasad L

更新日期：2004-06-01 00:00:00

abstract：:The beta-propeller architecture observed in protein tertiary structure and classified into the five different types according to number of 'blades' (or beta-sheets) and a sixth type classified according to the secondary structure composition of the blades (the beta beta alpha beta-molecular unit) is characterized by v...

journal_title：International journal of biological macromolecules

authors： Guruprasad K,Dhamayanthi P

更新日期：2004-04-01 00:00:00

abstract：:The rates of degradation, catalysed by Zn(2+), of four classical penicillins-amoxicillin, ampicillin and penicillins G and V-were followed at 20 degrees C in methanol by spectrophotometric assays. Kinetic schemes of the reactions of degradation catalysed by Zn(2+) ions were analogous to those given previously for the ...

journal_title：International journal of biological macromolecules

authors： Navarro PG,Blázquez IH,Osso BQ,Martínez de las Parras PJ,Puentedura MI,García AA

更新日期：2003-12-01 00:00:00

abstract：:A polysaccharide was isolated from the sheath of a sheathed bacterium, Sphaerotilus natans. The sheath polysaccharide (SPS) was composed of D-glucose and D-(N-acetyl)galactosamine in molar ratios of 1:4. Methylation linkage analysis revealed the presence of the residues of 4-linked glucose, 4-linked (N-acetyl)galactos...

journal_title：International journal of biological macromolecules

authors： Takeda M,Nakamori T,Hatta M,Yamada H,Koizumi J

更新日期：2003-12-01 00:00:00

abstract：:Ataxia telangiectasia (AT) is an autosomal recessive disorder characterized by numerous clinical and cellular features. The pleiotropic nature of the AT syndrome attests to the multiple roles of ATM, the protein codified by the gene altered in AT patients. We investigated if different mutations of ATM could reflect on...

journal_title：International journal of biological macromolecules

authors： Grattarola M,Spaggiari S,Chessa L,Savio C,Nicolini C,Vergani L

更新日期：2003-11-01 00:00:00

abstract：:Chitosan and heparin were covalently immobilized onto a poly(lactic acid-co-glycolic acid) (PLGA) surface using N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide (EDC), N-hydroxysuccinimide (NHS) in a 2-morpholinoethane sulfonic acid (MES) buffer system. The properties of the modified PLGA surface and the control were in...

journal_title：International journal of biological macromolecules

authors： Wang XH,Li DP,Wang WJ,Feng QL,Cui FZ,Xu YX,Song XH

更新日期：2003-11-01 00:00:00

abstract：:Contractile filaments in skeletal muscle are moved by less than 2nm for each ATP used. If just one cross-bridge is attached to each thin filament at any instant then this distance represents the fundamental myosin cross-bridge step size (i.e. the distance one cross-bridge moves a thin filament in one ATP-splitting cyc...

journal_title：International journal of biological macromolecules

authors： Barclay CJ

更新日期：2003-09-01 00:00:00

abstract：:Silk sericin (SS) possessing moisture-retaining property was reacted with activated poly(ethylene glycol) (PEG) to obtain self-assembled SS nanoparticles. The aliphatic and aromatic hydroxyl groups of serine and tyrosine residues as the reaction sites in SS were clarified by amino acid analysis and 1H NMR spectroscopy...

journal_title：International journal of biological macromolecules

authors： Cho KY,Moon JY,Lee YW,Lee KG,Yeo JH,Kweon HY,Kim KH,Cho CS

更新日期：2003-03-01 00:00:00

abstract：:Telomeric guanine-rich sequence can adopt quadruplex structures that are important for their biological role in chromosomal stabilisation. G quartets are characterised by the cyclic hydrogen bonding of four guanine bases in a coplanar arrangement and their stability is ion-dependent. In this work we compare the stabil...

journal_title：International journal of biological macromolecules

authors： Petraccone L,Erra E,Nasti L,Galeone A,Randazzo A,Mayol L,Barone G,Giancola C

更新日期：2003-01-15 00:00:00

abstract：:A fraction of low mobility group (LMG) nonhistone protein designated LMG(160) was isolated from rat liver chromatin by preparative gel electrophoresis and its interaction with DNA was studied using thermal denaturation and DNA-cellulose affinity chromatography techniques. The results showed that LMG(160) with an isoel...

journal_title：International journal of biological macromolecules

authors： Fallah S,Rabbani A

更新日期：2003-01-15 00:00:00

abstract：:Attenuated total reflectance-Fourier transform infrared (ATR-FTIR) was used to study the external regions of starch granules. Native starches (wheat, potato, maize, waxy maize and amylomaize) were analysed and compared to gelatinised and acid-hydrolysed starches. The IR spectra of potato and amylomaize starches were c...

journal_title：International journal of biological macromolecules

authors： Sevenou O,Hill SE,Farhat IA,Mitchell JR

更新日期：2002-12-20 00:00:00

abstract：:A deletion mutant of the transcarboxylase biotin carrier protein was completely labeled with 13C and 15N. A multitude of 2D and 3D NMR spectra were recorded and assigned. An NMR solution structure was derived from the data and compared in detail with the recently published structure of the wild-type. It is shown that ...

journal_title：International journal of biological macromolecules

authors： Jank MM,Sadowsky JD,Peikert C,Berger S

更新日期：2002-10-01 00:00:00

abstract：:Accommodation in the mammalian lens requires flexure of lens fibres and some redistribution of their contents involving limited viscous flow. Shear-dependent viscosity of bovine alpha-crystallin solutions was determined with the Contraves Low-Shear Rheometer between 4.4 and 347 mg ml(-1), and at 15.5, 25, 30, and 37 d...

journal_title：International journal of biological macromolecules

authors： Tiffany JM,Koretz JF

更新日期：2002-06-18 00:00:00

abstract：:Polyhydroxyalkanoate (PHA) synthase activity in Synechocystis sp. PCC6803 was increased two-fold by introducing the PHA biosynthetic genes of Ralstonia eutropha. The resulting recombinant Synechocystis sp. PCC6803 strain was subjected to conditions that favor PHA accumulation and the effects of various carbon sources ...

journal_title：International journal of biological macromolecules

authors： Sudesh K,Taguchi K,Doi Y

更新日期：2002-04-08 00:00:00

abstract：:In our earlier communication on urea denaturation of bovine serum albumin (BSA), we showed significant unfolding of domain III along with domain I prior to intermediate formation around 4.6-5.2 M urea based on the binding results of domain specific ligands:chloroform, bilirubin and diazepam for domains I, II and III, ...

journal_title：International journal of biological macromolecules

authors： Tayyab S,Ahmad B,Kumar Y,Khan MM

更新日期：2002-03-08 00:00:00

abstract：:From a set of mixed carbon sources, 5-phenylvaleric acid (PV) and octanoic acid (OA), polyhydroxyalkanoic acid (PHA) was separately accumulated in the two pseudomonads Pseudomonas putida BM01 and Pseudomonas citronellolis (ATCC 13674) to investigate any structural difference between the two PHA accumulated under a sim...

journal_title：International journal of biological macromolecules

authors： Chung DM,Choi MH,Song JJ,Yoon SC,Kang IK,Huh NE

更新日期：2001-12-10 00:00:00

abstract：:To evaluate the structural influence of the DNA phosphate backbone on the activity of Escherichia coli DNA topoisomerase I, modified forms of oligonucleotide dA(7) were synthesized with a chiral phosphorothioate replacing the non-bridging oxygens at each position along the backbone. A deoxy-iodo-uracil replaced the 5'...

journal_title：International journal of biological macromolecules

authors： Roche CJ,Tse-Dinh YC

更新日期：2001-10-22 00:00:00

abstract：:Structural characteristics and thermal and solution properties of the regenerated silk fibroin (SF) prepared from formic acid (FU) were compared with those of SF from water (AU). According to the turbidity and shear viscosity measurement, SF formic acid solution was stable and transparent, no molecular aggregations oc...

journal_title：International journal of biological macromolecules

authors： Um IC,Kweon HY,Park YH,Hudson S

更新日期：2001-08-20 00:00:00

abstract：:Effects of water activity (aW) and solvent ordering were separately analyzed on the thermal unfolding of lysozyme and alpha-chymotrypsinogen A, and also on the thermal deactivation of yeast alcohol dehydrogenase (YADH) in aqueous solutions with various additives. With the coexistence of additives, water activity was t...

journal_title：International journal of biological macromolecules

authors： Matsue S,Fujii T,Miyawaki O

更新日期：2001-06-12 00:00:00

abstract：:The interaction of an amino-terminal-truncated 139 amino-acids form of human acidic fibroblast growth factor with myo-inositol hexasulphate and low molecular weight (3500 g mol(-1)) heparin has been studied by isothermal titration calorimetry, differential scanning calorimetry and Fourier transform infrared spectrosco...

journal_title：International journal of biological macromolecules

authors： Guzmán-Casado M,Cardenete A,Giménez-Gallego G,Parody-Morreale A

更新日期：2001-04-12 00:00:00

abstract：:The structure of a ubiquitin-like protein, small ubiquitin-related modifier-1 (SUMO-1), was earlier determined using homonuclear nuclear magnetic resonance (NMR) spectroscopy, since the spectral quality of the protein was not suitable for heteronuclear NMR data collection. In this study, a slightly different construct...

journal_title：International journal of biological macromolecules

authors： Jin C,Shiyanova T,Shen Z,Liao X

更新日期：2001-03-14 00:00:00

abstract：:The solvent accessible surface area (ASA) of the polysaccharides, namely (i) carrageenan (1CAR); (ii) agarose (1AGA); (iii) guaran (GUR); (iv) capsular polysaccharide (1CAP); and (v) hyaluronan (1HUA), have been computed using the solvent accessibility technique of Lee and Richards. The results show that the average v...

journal_title：International journal of biological macromolecules

authors： Kaliannan P,Gromiha MM,Elanthiraiyan M

更新日期：2001-01-10 00:00:00

abstract：:Using thermal denaturation and fluorescence spectroscopy, we have investigated the interaction of antitumor antibiotic, daunomycin, with calf thymus histone H(1) under several ionic strengths. The results show that daunomycin binds to histone H(1) and increases its melting temperature. Increasing ionic strength elevat...

journal_title：International journal of biological macromolecules

authors： Zargar SJ,Rabbani A

更新日期：2000-10-10 00:00:00

abstract：:Apart from a few experimental studies muscle viscosity has not received much recent analytical attention as a determinant of the contractile process. This is surprising, since any muscle cell is 80% water, and may undergo large shape changes during its working cycle. Intuitively one might expect the viscosity of the s...

journal_title：International journal of biological macromolecules

authors： Elliott GF,Worthington CR

更新日期：2000-08-28 00:00:00

abstract：:Levans produced by several Zymomonas mobilis strains were classified by their viscosity average molecular weight and tested against sarcoma 180. Measurements of the samples' polydispersity were carried out to characterise the molecular weight distribution. The antitumour activities of levan samples were plotted agains...

journal_title：International journal of biological macromolecules

authors： Calazans GMT,Lima RC,de França FP,Lopes CE

更新日期：2000-07-12 00:00:00

abstract：:Results of small angle laser light scattering experiments carried out on polystyrene latex beads coated with lysate of Plasmodium falciparum (Pf) antigen (Ag) and human seropositive sera (10:1 ratio) are reported for various NaCl concentrations (0-300 mM). The protein coated beads showed time-dependent coagulation. Th...

journal_title：International journal of biological macromolecules

authors： Bohidar HB,Bhakat P,Sharma J,Saxena A

更新日期：2000-04-12 00:00:00

abstract：:Annealing processes, involving specific heating protocols, have been used by man for centuries to impart desirable properties to materials - especially metals and particularly tools and weapons. The terminology has also been applied to biopolymers such as starches, where the effects of the processing have been known f...

journal_title：International journal of biological macromolecules

authors： Tester RF,Debon SJ

更新日期：2000-03-16 00:00:00

abstract：:Poly-N-isopropylacrylamide (PNIPAM) is a chemical isomer of poly-leucine, having the polar peptide group in the side-chain rather than in the backbone. It has been demonstrated experimentally that PNIPAM dissolved in aqueous solution undergoes a collapse transition from coil to globule on increasing temperature above ...

journal_title：International journal of biological macromolecules

authors： Graziano G

更新日期：2000-03-16 00:00:00

abstract：:High-resolution fibre neutron diffraction data were recorded from cellulose samples on a D19 diffractometer at the Institut Laue-Langevin (Grenoble). Highly crystalline cellulose I samples from Cladophora (cellulose I alpha + I beta) or Halocynthia (cellulose I beta) origin were prepared in the form of oriented films....

journal_title：International journal of biological macromolecules

authors： Nishiyama Y,Okano T,Langan P,Chanzy H

更新日期：1999-12-01 00:00:00

abstract：:The role of both the length of saccharide chain and reducing terminals in the heparin molecule in causing oxidative effects on proteins was investigated by employing unfractionated and low-molecular-mass heparins (LMMH), with both intact and reduced reducing terminals on bovine trypsin. The effects of heparin were fou...

journal_title：International journal of biological macromolecules

authors： Finotti P,Pagetta A,Corvaja C

更新日期：1999-11-01 00:00:00

abstract：:The sequence distributions of two microbial copolyesters obtained by fermentation of Rhodospirillum rubrum, grown with 3-hydroxyhexanoic or 3-hydroxyheptanoic acids, were determined by analyzing the oligomers prepared by partial pyrolysis or partial methanolysis of these copolyesters using fast atom bombardment mass s...

journal_title：International journal of biological macromolecules

authors： Ballistreri A,Giuffrida M,Impallomeni G,Lenz RW,Fuller RC

更新日期：1999-11-01 00:00:00

abstract：:A new non-sulphated acidic polysaccharide with an average molecular mass of 55 kDa was isolated from squid pen case after papain digestion and beta-elimination. This polysaccharide contains mainly L-iduronic acid, D-glucuronic acid, D-galactosamine, D-glucosamine and significant amounts of neutral sugars as glucose, g...

journal_title：International journal of biological macromolecules

authors： Theocharis AD,Karamanos NK,Tsegenidis T

更新日期：1999-10-01 00:00:00

abstract：:Previous investigations on the role of the polymerase in the synthesis of poly-3-hydroxybutyrate (PHB) are reviewed, and the results from earlier in vitro studies on the activity and selectivity of the polymerase of Alcaligenes eutrophus are discussed. In the present study the effect of glycerol on stabilizing the pol...

journal_title：International journal of biological macromolecules

authors： Lenz RW,Farcet C,Dijkstra PJ,Goodwin S,Zhang S

更新日期：1999-06-01 00:00:00

abstract：:The PHA-degrading isolate, strain P37C, was enriched from residential compost for its ability to hydrolyze the medium chain length PHA, poly(beta-hydroxyoctanoate) (PHO). It was subsequently found to grow on a wide range of PHAs, including both short chain length and medium chain length PHAs. The isolate was identifie...

journal_title：International journal of biological macromolecules

authors： Quinteros R,Goodwin S,Lenz RW,Park WH

更新日期：1999-06-01 00:00:00

abstract：:Two types of the biological macromolecules poly(R-3-hydroxyalkanoates) have been identified: the high-molecular-weight microbial storage material (sPHA) and a short-chain variety, consisting of butyrate and valerate residues, complexed with other biomacromolecules such as calcium polyphosphate or proteins (cPHB/PHV). ...

journal_title：International journal of biological macromolecules

authors： Seebach D,Fritz MG

更新日期：1999-06-01 00:00:00